In control animals, MIG-14 was visible throughout the cell and at the plasma membrane. However, in animals that were treated with RNAi against the AP-2 subunits
dpy-23,
apa-2, or
aps-2 or the shared AP-2 and AP-1 subunit
apb-1 (Boehm and Bonifacino, 2001), there was a striking accumulation of MIG-14 on the cell membrane, indicating that internalization of MIG-14 is impaired in the absence of a functional AP-2 complex.