"worms that express the green fluorescent protein (GFP) under the regulation of the
hsp-16.2 promoter (strain CL2070). CL2070 worms were grown on control bacteria harboring the empty vector (EV). The animals were then divided to identical groups and treated at days 1 and 2 of adulthood for 3 h with the chemical vehicle of NT219 [Fig. 2A, lane 1 (Ve)], or with 150, 300, 600 or 900 lM NT219 (lanes 3-6, respectively). An identical worm group was grown from hatching on
daf-2 RNAi bacteria (lane 2). At day 2 of adulthood, all worm groups were exposed to 33C for 3 h and harvested, and GFP levels were analyzed by WB. We found that NT219 increased the
hsp-16.2 induction levels in heat-stressed animals in a dose-dependent manner. While treatment with 150 lM NT219 had no effect on the level of GFP, 300 lM had a moderate effect and 600 lM NT219 remarkably increased the level of GFP to be similar to that of
daf-2 RNAi-treated worms. Treatment with 900 lM NT219 had lower effect than that of 600 lM of the drug. Three independent repeats confirmed these observations (Fig. 2B and S2)."