"we designed an RNAi suppressor screen to identify genes whose knockdown could restore tunicamycin
ckb-2p::gfp activation following ER stress in a
cdc-48.2(-/-) mutant background ([4]; Fig 1C)... Two-hundred and forty-one RNAi clones synergized with
cdc48.2(-/-) to decrease
ckb-2p::gfp expression in our primary screen (mean Z-score value less than -1.5, or one of the two independent Z-scores less than -3) (Fig 1C). Of these, 59 clones significantly decreased GFP fluorescence below 0.75-fold (P < 0.05) (Fig 1D, Supplementary Table S2)."