"we measured fluorescence intensity in
cdc-48.2(-/-);
ckb-2p::gfp worms fed with candidate RNAi clones and treated either with tunicamycin or vehicle (DMSO; Fig 1E). Seventy-seven RNAi clones showing a similar increase in the fluorescence ratios under both conditions were considered ER-stress independent and not further analyzed (Fig 1F, Supplementary Table S3)."