ASJ minimal promoter : ssu-1

Using sequential promoter deletions authors found a minimal region of 334 bp upstream of the ATG codon in the ssu-1 promoter that drives expression in ASJ neurons.

ASJ minimal promoter : trx-1

trx-1 promoter analyses identified a minimal region of 116 bp (positioned at - 200 to -84 bp upstream of the trx-1b isoform ATG codon) that drives GFP expression in ASJ, thus containing the putative cis-regulatory motif responsible for ASJ expression.

Transcription factor binding site : ssu-1

The deletion or the mutation of the individual 6 bp elements of the ASJ motif in the ssu-1 promoter robustly decreases gene ex- pression in ASJ, indicating that the ASJ motif is also functional in ssu-1.

Transcription factor binding site : ssu-1

The deletion or the mutation of the individual 6 bp elements of the ASJ motif in the ssu-1 promoter robustly decreases gene ex- pression in ASJ, indicating that the ASJ motif is also functional in ssu-1.

sptf-1 : ssu-1

sptf-1 RNAi fully abolished Pssu-1::gfp expression in ASJ neurons. Of note, ASJ labeling is abolished not only in adult animals but also in embryos and larvae, indicating that sptf-1 deficiency impairs ASJ initiation of gene expression, rather than ASJ gene expression maintenance. sptf-1(tm784) arrested L1 larvae carrying the Pssu-1::gfp transgenes are devoid of ASJ fluorescence.

sptf-1 : trx-1

sptf-1 RNAi fully abolished Ptrx-1::gfp expression in ASJ neurons. Of note, ASJ labeling is abolished not only in adult animals but also in embryos and larvae, indicating that sptf-1 deficiency impairs ASJ initiation of gene expression, rather than ASJ gene expression maintenance. sptf-1(tm784) arrested L1 larvae carrying the Ptrx-1::gfp transgenes are devoid of ASJ fluorescence.

bipartite promoter : ssu-1

Deletion of -234 to -208 bp upstream of the ssu-1 ATG codon region in the ssu-1 promoter or mutation of each individual element of the bipartite ASJ motif, disrupted expression in ASJ.

dietary deprivation : trx-1

Dietary deprivation significantly increased Ptrx-1::GFP fluorescence in ASJ neurons of aging adults.

cmk-1 : daf-28

A daf-28p::gfp reporter is expressed strongly in both ASI and ASJ neurons in fed wild- type larvae. In fed cmk-1 mutant larvae grown on live bacteria without exogenous pheromone, daf-28p::gfp expression was strongly decreased in ASJ and more weakly affected in ASI.

Dauer pheromone mixture : ins-6

crude dauer pheromone mixture downregulates ins-6p::mCherry in ASI, while dauer pheromone and the dauer state induces ins-6p::mCherry in ASJ