"We next examined presynaptic UNC-13 localization in order to monitor the regulation of the
dgk-1/egl-30 pathway by AEX-1 (Nurrish et al., 1999) (Figure 6). In wild-type animals, UNC-13::GFP accumulated at a density of 1.7 puncta/10um, while, in the
aex-1(
sa9) mutants, UNC-13::GFP density was significantly reduced and more diffusely distributed (0.5 puncta/10um). This phenotype was rescued by introduction of both
aex-1 genomic and Pmyo-3AEX-1(+) (muscle-specific expression), but not by Paex-3::AEX-1(+) (neural and intestinal expression) (Figure 6E)... Neural EGL-30(R243Q gain-of-function) also suppressed the low density of UNC-13 accumulation in the
aex-1(
sa9) mutant (Figure 6E), confirming that
egl-30 is epistatic to
aex-1."