gfp expression driven under only 165 bp of the regulatory sequence of
srh-234 (
srh-234p::gfp) is strongly expressed in ADL in fed animals, but when animals were starved for long-periods of time (.6 hours),
srh-234p::gfp was significantly downregulated. Authors further confirmed the starvation-induced change in expression by examining the endogenous levels of
srh-234 with help of qRT-PCRs, and found that the transcript levels of
srh-234 were similarly downregulated but not abolished in starved animals.