Semi-quantitative RT-PCR was performed to compare levels of
daf-15 mRNA in
daf-2(
e1370) (in which DAF-16 is activated) and
daf-16(mgDf47);
daf-2(
e1370), in which there is no DAF-16 activity. The
daf-15 mRNA level was reduced in
daf-2 compared with
daf-16;
daf-2, indicating that DAF-16 negatively regulates the expression of
daf-15.