To examine whether UNC-3 can physically bind these sites, we performed Within this 1.2 kb
egl-1 fragment, we identified two putative COE binding sites (sites 1 and 2) and a less stringent binding site (site 3), which are conserved among the C. elegans, C. remaneii, C. briggsae and C. brenneri genomes. ChIP analysis with a FLAG-tagged full-length UNC-3 fusion protein. Compared with other regions tested, a strong binding signal was detected around sites 1 and 2.