rfip-1p_d::gfp expression disappeared in
egl-5-deficient PLM neurons, and the reporter was ectopically expressed in ALM neurons when
egl-5 was misexpressed. Similarly, smFISH confirmed that the number of
rfip-1 transcripts decreased markedly in PLM neurons that lacked
egl-5. On the other hand,
rfip-1 mRNA levels increased significantly in ALM neurons expressing
egl-5. These re- sults suggest that EGL-5 activate
rfip-1 transcription to induce the growth of the posterior neurite in PLM neurons.