In
daf-16(mgDf47);
daf-7(+), GFP::DAF-16B was predominantly cytoplasmic. In a
daf-16(mgDf47);
daf-7(
m62) background under dauer-inducing conditions, GFP::DAF-16B was almost exclusively localized in the nucleus throughout the animal but only during the L2d predauer stage. At all other stages in the
daf-7 mutant, including in the dauer stage, GFP::DAF-16 was largely excluded from the nucleus.