- WBPaper00065623:33
CellRanger, DecontX, Monocle3, Louvain algorithm.
Single-cell RNA-Seq cell group 33 expressed in: AS; DA_VA; DB_VB; and SAB.
- WBPaper00056293:rpl-5(0)-rpl-33(0)_downregulated
Limma Package. When generating the contrast matrix in limma: wildtype data was counted as wild-type and rpl-33 & rpl-5 data were both counted as RP null data. For this reason there is only one fold change value per gene as calculated by the difference between RP null RNA and wild-type RNA.
Transcripts that showed significantly decreased expression in rpl-5(cc5998) vs wild type (PD1074) and rpl-33(cc2558) vs wild type (PD1074) animals.
- WBPaper00056293:rpl-5(0)-rpl-33(0)_upregulated
Limma Package. When generating the contrast matrix in limma: wildtype data was counted as wild-type and rpl-33 & rpl-5 data were both counted as RP null data. For this reason there is only one fold change value per gene as calculated by the difference between RP null RNA and wild-type RNA.
Transcripts that showed significantly increased expression in rpl-5(cc5998) vs wild type (PD1074) and rpl-33(cc2558) vs wild type (PD1074) animals.
- WBPaper00049942:HeatShock_upregulated_hsf-1(+)
Transcripts that were differentially expressed in different conditions, compared to the hsf-1(+);-HS control, were determined with CuffDiff, which uses the Benjamini-Hochberg correction for multiple testing to obtain the q-value (the FDR-adjusted the p-value).
Transcripts that showed significantly increased expression in animals injected with control vector, after heat shock at 33 C for 30min, comparing to without heat shock.
- WBPaper00049942:HeatShock_downregulated_hsf-1(+)
Transcripts that were differentially expressed in different conditions, compared to the hsf-1(+);-HS control, were determined with CuffDiff, which uses the Benjamini-Hochberg correction for multiple testing to obtain the q-value (the FDR-adjusted the p-value).
Transcripts that showed significantly decreased expression in animals injected with control vector, after heat shock at 33 C for 30min, comparing to without heat shock.
- WBPaper00049942:HeatShock_upregulated_hsf-1(RNAi)
Transcripts that were differentially expressed in different conditions, compared to the hsf-1(+);-HS control, were determined with CuffDiff, which uses the Benjamini-Hochberg correction for multiple testing to obtain the q-value (the FDR-adjusted the p-value).
Transcripts that showed significantly increased expression in hsf-1(RNAi) animals, after heat shock at 33 C for 30min, comparing to in hsf-1(RNAi) animals without heat shock.
- WBPaper00049942:HeatShock_downregulated_hsf-1(RNAi)
Transcripts that were differentially expressed in different conditions, compared to the hsf-1(+);-HS control, were determined with CuffDiff, which uses the Benjamini-Hochberg correction for multiple testing to obtain the q-value (the FDR-adjusted the p-value).
Transcripts that showed significantly decreased expression in hsf-1(RNAi) animals, after heat shock at 33 C for 30min, comparing to in hsf-1(RNAi) animals without heat shock.