- WBPaper00035654:pdr-1(gk448)_regulated
TIGR Spotfinder software was used to filter unreliable spots based on Otsu's method algorithm and to convert good spots in microarray images to numerical form. Raw signal values were analyzed using mean centering to normalize signal values between channels. Signals from Alexa Fluor Dye Control probes were excluded from normalization process due their oversaturation leaving only probe intensities from C. elegans miRNAs to use in normalization. Signal intensity ratios were calculated for each signal pair yielding fold change values and p values for each miRNAs from the strain of investigation. A cutoff of 1.4 was used to detect modestly changing miRNAs.
Genes that differentially expressed in pdr-1(gk448) comparing with N2 at L4 larva.
- WBPaper00045217:prg-1_progressively_regulated
Genes with more than 2-fold change in expression level are considered differentially expressed.
Genes altered by more than 2-Fold in late versus early generation prg-1 mutants and prg-1; daf-2 mutants. Samples include prg-1(pk2290), prg-1(n4357), prg-1(tm872), prg-1(pk2290); daf-2(e1368), prg-1(pk2290); daf-2(e1370), prg-1(tm872); daf-2(e1370), prg-1(tm872); daf-2(m41).
- WBPaper00059609:malt-1_ilc-17.1_nfki-1_downregulated
Cufflinks (v2.2.1). q < 0.05.
Transcripts that showed significantly decreased expression in malt-1(db1194);npr-1(ad609) animals, in npr-1(ad609) ilc-17.1(tm5218) animals, and in npr-1(ad609) nfki-1(db1198) animals, comparing to the wild type control npr-1(ad609).
- WBPaper00059609:malt-1_ilc-17.1_nfki-1_upregulated
Cufflinks (v2.2.1). q < 0.05.
Transcripts that showed significantly increased expression in malt-1(db1194);npr-1(ad609) animals, in npr-1(ad609) ilc-17.1(tm5218) animals, and in npr-1(ad609) nfki-1(db1198) animals, comparing to the wild type control npr-1(ad609).
- WBPaper00047132:skn-1(RNAi)_downregulated_glp-1(ts)-dependent
Fold change > 4.0 for glp-1(ts) regulated genes, and fold change < 0.67 for skn-1(RNAi) regulated genes.
Genes with < 0.67 fold decreased expression in skn-1(RNAi) animals comparing to in N2 control at 25 centigrade. Fold change = glp-1(ts) + skn-1 RNAi vs. glp-1(ts) + vector
- WBPaper00050094:glp-1(gf)_downregulated
Fold change > 2.
Transcripts that showed significantly decreased expression in the dissected gonads of gld-2(q492) gld-1(q485) (I); glp-1(ar202)(III) (glp-1(gf) NOTCH ON), comparing to in the dissected gonads of gld-2(q492) gld-1(q485) (I); glp-1(e2144) (glp-1(lf) NOTCH OFF)