- WBPaper00035227:bio-electrospray_regulated
Differences between treatments were visualized by principal component analysis (PCA) plotting with MeV/TM4. Data were initially filtered out for missing values and then subjected to a CLEAR test that combines differential expression and variability using the GEPAS web server at http://www.gepas.org. In our case, the false discovery rate was set to a stringent level of 5 per cent.
Genes regulated by bio-electrospray.
- WBPaper00024704:Soluble_protein
N.A.
Soluble proteins identified according to HPLC electrospray ionization (ESI)-MS-MS, using the method in which the protein mixtures were first derivatized with a water-soluble fluorogenic reagent, SBD-F or 7-chloro-4-(dimethylaminoethylaminosulfonyl)-2,1,3-benzoxadiazole (DAABD-Cl).
- WBPaper00049098:hrde-1(tm1200)-induced_p15C
The Bio-conductor R package of DEseq was used for this analysis. hrde-1 vs. N2 expression level fold change >= 2.0, FDR <= 0.1.
Transcripts that were induced by hrde-1(tm1200) at p15C comparing to in N2 animals.
- WBPaper00049098:hrde-1(tm1200)-induced_23C
The Bio-conductor R package of DEseq was used for this analysis. hrde-1 vs. N2 expression level fold change >= 2.0, FDR <= 0.1.
Transcripts that were induced by hrde-1(tm1200) at 23C comparing to in N2 animals.
- WBPaper00049098:hrde-1(tm1200)-repressed_23C
The Bio-conductor R package of DEseq was used for this analysis. hrde-1 vs. N2 expression level fold change >= 2.0, FDR <= 0.1.
Transcripts that were repressed by hrde-1(tm1200) at 23C comparing to in N2 animals.
- WBPaper00049098:hrde-1(tm1200)-repressed_15C
The Bio-conductor R package of DEseq was used for this analysis. hrde-1 vs. N2 expression level fold change >= 2.0, FDR <= 0.1.
Transcripts that were repressed by hrde-1(tm1200) at 15C comparing to in N2 animals.
- WBPaper00049098:hrde-1(tm1200)-induced_15C
The Bio-conductor R package of DEseq was used for this analysis. hrde-1 vs. N2 expression level fold change >= 2.0, FDR <= 0.1.
Transcripts that were induced by hrde-1(tm1200) at 15C comparing to in N2 animals.
- WBPaper00049098:hrde-1(tm1200)-repressed_p15C
The Bio-conductor R package of DEseq was used for this analysis. hrde-1 vs. N2 expression level fold change >= 2.0, FDR <= 0.1.
Transcripts that were repressed by hrde-1(tm1200) at p15C comparing to in N2 animals.
- WBPaper00049098:heat-induced_N2
The Bio-conductor R package of DEseq was used for this analysis. Heat-induced genes were defined as genes with at least twofold increase in their mean mRNA expression levels at 23C over the ones at 15C (FDR <= 0.1) in both biological repeats. Heat-repressed genes were defined as genes with at least two-fold decreases in their mean mRNA expression levels at 23C over the ones at 15C (FDR <= 0.1) in both biological repeats.
Transcripts that were induced in N2 animals by multigenerational heat stress.
- WBPaper00049098:heat-repressed_N2
The Bio-conductor R package of DEseq was used for this analysis. Heat-induced genes were defined as genes with at least twofold increase in their mean mRNA expression levels at 23C over the ones at 15C (FDR <= 0.1) in both biological repeats. Heat-repressed genes were defined as genes with at least two-fold decreases in their mean mRNA expression levels at 23C over the ones at 15C (FDR <= 0.1) in both biological repeats.
Transcripts that were repressed in N2 animals by multigenerational heat stress.