Expressed transcripts were identified on the basis of a Present call in 3 out of 4 N2 experiments as determined by Affymetrix MAS 5.0.
Genes expressed in N2.
Genes that showed significantly changed expression in oxidative stressed N2 animals caused by 10mM sodium arsenite (NaAsO2) comparing to in unstressed N2.
Fold change > 4.0 for glp-1(ts) regulated genes, and fold change < 0.67 for skn-1(RNAi) regulated genes.
Genes with >0.67 fold decreased expression in skn-1(RNAi) animals comparing to in N2 control at 25 centigrade. fold change = N2 + skn-1 RNAi vs. N2 + vector.
Transcripts that showed significantly decreased expression after N2 L4 animals were infected by P. aeruginosa (PA14) bacteria for 4 hours at 25C, comparing to N2 animals grown in OP50.
Transcripts that showed significantly increased expression after N2 L4 animals were infected by P. aeruginosa (PA14) bacteria for 4 hours at 25C, comparing to N2 animals grown in OP50.
Genes with significant upregulation following hypoxia in an ELT-2-dependent manner. RNAseq were performed on normoxia N2, hypoxia N2, normoxia elt-2(RNAi) and hypoxia elt-2(RNAi) animals.
Genes that showed significantly decresed level of post-transcriptional processing in daf-2(e1370)rsks-1(ok1255) vs N2 animals. Diferential Polysome Asso-ciation Ratio (DPAR) = log2 fold changes of the ratio (daf-2 rsks-1 polysomal associated mRNA / daf-2 rsks-1 total mRNA) / (N2 polysomal associated mRNA / N2 total mRNA).