WBPaper00041174:environmental

two-way ANOVA Genes with variation in expression level across conditions (control, 25C, High-pH-Salt, Liquid, infection).

WBPaper00060434:pH4.33_vs_pH6.33_uprgulated

DESeq2 (1.16.1). The Benjamini and Hochberg's approach was used to adjust the resulting P-values to control the false discovery rate. Corrected value of P < 0.05 and fold change > 2 were set as the threshold for significantly differential expression. Transcripts that showed significantly increased expression after 3 hours of exposure to acidic environment (PH 4.33), comparing to control animals exposed to PH 6.33 environment.

WBPaper00060434:pH4.33_vs_pH6.33_downrgulated

DESeq2 (1.16.1). The Benjamini and Hochberg's approach was used to adjust the resulting P-values to control the false discovery rate. Corrected value of P < 0.05 and fold change > 2 were set as the threshold for significantly differential expression. Transcripts that showed significantly decreased expression after 3 hours of exposure to acidic environment (PH 4.33), comparing to control animals exposed to PH 6.33 environment.

WBPaper00041174:genotypic_prime

two-way ANOVA Genes with significant variation in expression level only across genotypes(AB2, CB4856, RC301, CB4857, N2, HC445), but not environments (control, 25C, High-pH-Salt, Liquid, infection).

WBPaper00041174:environmental_prime

two-way ANOVA Genes with significant variation in expression level only across environments(control, 25C, High-pH-Salt, Liquid, infection), but no genotypic variation (AB2, CB4856, RC301, CB4857, N2, HC445).