- WBPaper00059440:GLP-1_activated
edgeR fold change >= 2, FDR <= 0.05.
Transcripts activated by glp-1(ar202), a gain-of-function allele of glp-1, in the gld-2(q497) gld-1(q485) background at young adult stage, comparing to glp-1(q175) null allele.
- WBPaper00059440:GLP-1_represseded
edgeR fold change >= 2, FDR <= 0.05.
Transcripts repressed by glp-1(ar202), a gain-of-function allele of glp-1, in the gld-2(q497) gld-1(q485) background at young adult stage, comparing to glp-1(q175) null allele.
- WBPaper00059763:neuron_acr-2(n2420gf)_regulated
DESeq2, p-value < 0.05. Identification of expressed genes in wildtype was determined using Cufflinks. Expressed genes were selected by filtering for genes with an FPKM>10 in both replicates.
Transcripts that showed significantly altered expression in acr-2(n2420) gain-of-function animals comparing to in wild type background during neuron-specic RNASeq analysis via FACS of Pacr-2::gfp reporter juIs14.
- WBPaper00041207:CE_dauer_up
The weight parameters were optimized based on MA-plots such that spike-in controls show their expected fold change values. lmFit function was used to fit a linear model to probe intensities across arrays, and differential expression was calculated by empirical Bayes method using the eBayes function. Control of FDR was employed as correction for multiple testing.
Genes up regulated in the dauer versus dauer-exit worms.
- WBPaper00041207:CE_dauer_down
The weight parameters were optimized based on MA-plots such that spike-in controls show their expected fold change values. lmFit function was used to fit a linear model to probe intensities across arrays, and differential expression was calculated by empirical Bayes method using the eBayes function. Control of FDR was employed as correction for multiple testing.
Genes dowm regulated in the dauer versus dauer-exit worms.
- WBPaper00048762:FOG-1_associated
To identify transcripts enriched in RIP samples, background corrected and normalized data were analyzed with the Bioconductor package Siggenes (siggenes 1.38.0) using two-class significance analysis of microarrays (SAM function).
FOG-1associated mRNAs identified by RIP-ChIP
- WBPaper00048762:FOG-3_associated
To identify transcripts enriched in RIP samples, background corrected and normalized data were analyzed with the Bioconductor package Siggenes (siggenes 1.38.0) using two-class significance analysis of microarrays (SAM function).
FOG-1associated mRNAs identified by RIP-ChIP