- WBPaper00062354:H.zoospora_12h_regulated
Kalisto abundance files were converted and analysed using Sleuth in a R pipeline. Standard Sleuth protocols were used to calculate differential expression. P value < 0.01 and FDR < 0.01.
Transcripts that showed significantly altered expression after L4 N2 animals were exposed to omycete Haptoglossa zoospora for 12 hours.
- WBPaper00062354:H.zoospora_6h_regulated
Kalisto abundance files were converted and analysed using Sleuth in a R pipeline. Standard Sleuth protocols were used to calculate differential expression. P value < 0.01 and FDR < 0.01.
Transcripts that showed significantly altered expression after L4 N2 animals were exposed to omycete Haptoglossa zoospora for 6 hours.
- WBPaper00038421:egl-4(lf)_upregulated
Data was analyzed using the R statistical environment. CEL files resulting from array analysis were interpreted and normalized using RMA. The linear modeling package Limma was used to determine significant gene expression differences based on a moderated t-statistic.
Top 10 differentially expressed genes with expression repressed in egl-4(gf) and activated in egl-4(lf) animals.
- WBPaper00061479:hrde-1(tm1200)_upregulated
Salmon was used to map the mRNA-seq reads with the worm database WS268, and its output files were imported to DESeq2 in R. The differentially expressed genes were filtered by fold change more than 2 and adjusted p-value < 0.05. The scatter plots were generated by the plot function in R.
Transcripts that showed significantly increased expression in hrde-1(tm1200) in gonads dissected from 1-day old adult animals.
- WBPaper00061479:hda-1(ne4747)_upregulated
Salmon was used to map the mRNA-seq reads with the worm database WS268, and its output files were imported to DESeq2 in R. The differentially expressed genes were filtered by fold change more than 2 and adjusted p-value < 0.05. The scatter plots were generated by the plot function in R.
Transcripts that showed significantly increased expression in hda-1[KKRR] in gonads dissected from 1-day old adult animals.
- WBPaper00061479:prg-1(tm872)_upregulated
Salmon was used to map the mRNA-seq reads with the worm database WS268, and its output files were imported to DESeq2 in R. The differentially expressed genes were filtered by fold change more than 2 and adjusted p-value < 0.05. The scatter plots were generated by the plot function in R.
Transcripts that showed significantly increased expression in prg-1(tm872) in gonads dissected from 1-day old adult animals.
- WBPaper00061479:mep-1(ne4629)_upregulated
Salmon was used to map the mRNA-seq reads with the worm database WS268, and its output files were imported to DESeq2 in R. The differentially expressed genes were filtered by fold change more than 2 and adjusted p-value < 0.05. The scatter plots were generated by the plot function in R.
Transcripts that showed significantly increased expression in mep-1(ne4629[MEP-1-GFP-Degron]) in gonads dissected from 1-day old adult animals.
- WBPaper00061479:ubc-9(ne4833)_upregulated
Salmon was used to map the mRNA-seq reads with the worm database WS268, and its output files were imported to DESeq2 in R. The differentially expressed genes were filtered by fold change more than 2 and adjusted p-value < 0.05. The scatter plots were generated by the plot function in R.
Transcripts that showed significantly increased expression in ubc-9(ne4833[ubc-9(G56R)] in gonads dissected from 1-day old adult animals.
- WBPaper00061479:hda-1(ne4752)_upregulated
Salmon was used to map the mRNA-seq reads with the worm database WS268, and its output files were imported to DESeq2 in R. The differentially expressed genes were filtered by fold change more than 2 and adjusted p-value < 0.05. The scatter plots were generated by the plot function in R.
Transcripts that showed significantly increased expression in hda-1(ne4752[3xFLAG-Degron-HDA-1]) in gonads dissected from 1-day old adult animals.
- WBPaper00061479:hda-1(ne4748)_upregulated
Salmon was used to map the mRNA-seq reads with the worm database WS268, and its output files were imported to DESeq2 in R. The differentially expressed genes were filtered by fold change more than 2 and adjusted p-value < 0.05. The scatter plots were generated by the plot function in R.
Transcripts that showed significantly increased expression in hda-1[KKRR]-smo-1 in gonads dissected from 1-day old adult animals.