DESeq2 p-value <0.05, expression of hypoxia vs. normxia >= 2, expression in egl09(sa307) vs N2 < 2, expression of egl-9(sa307); hif-1(ia04) vs N2 <= 2.
Transcripts that showed significantly increased expression after hypoxia in an egl-9 / hif-1 independent manner.
Data was analyzed using the R statistical environment. CEL files resulting from array analysis were interpreted and normalized using RMA. The linear modeling package Limma was used to determine significant gene expression differences based on a moderated t-statistic.
Top 10 differentially expressed genes with expression repressed in egl-4(gf) and activated in egl-4(lf) animals.