- WBPaper00055311:ppa-daf-19_upregulated
Differentially expressed genes were identified by the program cuffdiff (version 2.2.1) from the comparison of both Ppa-daf-19 alleles with two wildtype control samples. The sets of up- and down-regulated genes (FDR corrected p-value <0.05).
Transcripts that showed significantly increased expression in ppa-daf-19(tu1035) and ppa-daf-19(tu1124) comparing to RS2333 wild type control samples.
- WBPaper00055311:ppa-daf-19_downregulated
Differentially expressed genes were identified by the program cuffdiff (version 2.2.1) from the comparison of both Ppa-daf-19 alleles with two wildtype control samples. The sets of up- and down-regulated genes (FDR corrected p-value <0.05).
Transcripts that showed significantly decreased expression in ppa-daf-19(tu1035) and ppa-daf-19(tu1124) comparing to RS2333 wild type control samples.
- WBPaper00053550:daf-19(m86)_downregulated_L1
BRB Array Tools were used to identify genes with statistically significant variation in expression. The probability threshold was set at a maximum of 0.05 (p-value <= 0.05) for genes to be considered statistically differentially expressed in wild-type and mutant populations. Genes with a signal variation of 1.5-fold or greater were selected for subsequent experiments. To reduce false discoveries, a class comparison test was conducted using a multivariate per mutation test with a confidence level of 97% (L1 analysis) and 90% (adults).
Genes that showed significantly decreased expression in daf-19(m86);daf-12(sa204) comparing to in daf-12(sa204), at L1 larva stage.
- WBPaper00053550:daf-19(m86)_upregulated_L1
BRB Array Tools were used to identify genes with statistically significant variation in expression. The probability threshold was set at a maximum of 0.05 (p-value <= 0.05) for genes to be considered statistically differentially expressed in wild-type and mutant populations. Genes with a signal variation of 1.5-fold or greater were selected for subsequent experiments. To reduce false discoveries, a class comparison test was conducted using a multivariate per mutation test with a confidence level of 97% (L1 analysis) and 90% (adults).
Genes that showed significantly increased expression in daf-19(m86);daf-12(sa204) comparing to in daf-12(sa204), at L1 larva stage.
- WBPaper00053550:daf-19(m86)_downregulated_3-fold-embryo
BRB Array Tools were used to identify genes with statistically significant variation in expression. The probability threshold was set at a maximum of 0.05 (p-value <= 0.05) for genes to be considered statistically differentially expressed in wild-type and mutant populations. Genes with a signal variation of 1.5-fold or greater were selected for subsequent experiments. To reduce false discoveries, a class comparison test was conducted using a multivariate per mutation test with a confidence level of 97% (L1 analysis) and 90% (adults).
Genes that showed significantly decreased expression in daf-19(m86);daf-12(sa204) comparing to in daf-12(sa204), at 3-fold embryo stage.
- WBPaper00053550:daf-19(m86)_upregulated_AdultDay2
BRB Array Tools were used to identify genes with statistically significant variation in expression. The probability threshold was set at a maximum of 0.05 (p-value <= 0.05) for genes to be considered statistically differentially expressed in wild-type and mutant populations. Genes with a signal variation of 1.5-fold or greater were selected for subsequent experiments. To reduce false discoveries, a class comparison test was conducted using a multivariate per mutation test with a confidence level of 97% (L1 analysis) and 90% (adults).
Genes that showed significantly increased expression in daf-19(m86);daf-12(sa204) comparing to in daf-12(sa204), at 2-day post-L4 adult hermaphrodite stage.
- WBPaper00053550:daf-19(m86)_downregulated_AdultDay2
BRB Array Tools were used to identify genes with statistically significant variation in expression. The probability threshold was set at a maximum of 0.05 (p-value <= 0.05) for genes to be considered statistically differentially expressed in wild-type and mutant populations. Genes with a signal variation of 1.5-fold or greater were selected for subsequent experiments. To reduce false discoveries, a class comparison test was conducted using a multivariate per mutation test with a confidence level of 97% (L1 analysis) and 90% (adults).
Genes that showed significantly decreased expression in daf-19(m86);daf-12(sa204) comparing to in daf-12(sa204), at 2-day post-L4 adult hermaphrodite stage.
- WBPaper00053550:daf-19(m86)_upregulated_3-fold-embryo
BRB Array Tools were used to identify genes with statistically significant variation in expression. The probability threshold was set at a maximum of 0.05 (p-value <= 0.05) for genes to be considered statistically differentially expressed in wild-type and mutant populations. Genes with a signal variation of 1.5-fold or greater were selected for subsequent experiments. To reduce false discoveries, a class comparison test was conducted using a multivariate per mutation test with a confidence level of 97% (L1 analysis) and 90% (adults).
Genes that showed significantly increased expression in daf-19(m86);daf-12(sa204) comparing to in daf-12(sa204), at 3-fold embryo stage.
- WBPaper00039866:ClassComp_daf-19_upregulated
BRB Array Tools (version 3.3) was subsequently used to identify genes with a statistically significant variation in expression when comparing between the two classes defined in this study. The probability threshold was set at a maximum of 0.05 (p-value <= 0.05) for genes to be considered statistically different. Genes with a signal variation of 1.5-fold or greater were selectively identified for use in all subsequent experiments. To reduce the chances of false discoveries, a class comparison test was conducted using a multivariate permutation test with the confidence level of 97%.
Candidate daf-19 up regulated genes with a statistically significant signal variation of 1.5-fold or greater. These were identified using a class comparisons tool from BRB Array Tools.
- WBPaper00039866:ClassComp_daf-19_downregulated
BRB Array Tools (version 3.3) was subsequently used to identify genes with a statistically significant variation in expression when comparing between the two classes defined in this study. The probability threshold was set at a maximum of 0.05 (p-value <= 0.05) for genes to be considered statistically different. Genes with a signal variation of 1.5-fold or greater were selectively identified for use in all subsequent experiments. To reduce the chances of false discoveries, a class comparison test was conducted using a multivariate permutation test with the confidence level of 97%.
Candidate daf-19 down regulated genes with a statistically significant signal variation of 1.5-fold or greater. These were identified using a class comparisons tool from BRB Array Tools.