The microarray data were subjected to the Robust Multichip Averaging (RMA) Algorithm by using Partek Software (Partek, Inc., St. Charles, Missouri). GeneSpringSoftware9.0(AgilentTechnologies) was used to perform hierarchial cluster analysis.
Genes that showed increased expression in ced-1(e1735) comparing with in N2.
The data were processed using Partek Genomics Suite, version 6.4 Partek Inc, St. Louis, MO. The robust multichip averaging algorithm was used to normalize and summarize the probe data into probe set expression values. Analysis of variance, fold-change, and false discovery rate (FDR) calculations were also performed using Partek H Genomics Suite TM version 6.5 (Copyright 2010 Partek Inc., St. Louis, MO, USA). Transcripts showing a corrected p-value of <= 0.05 and fold change <= -1.2 or >= 1.2 were considered differentially expressed between experimental treatments groups.
Genes induced over twofold following infection of C. elegans with hly(+) V. cholerae strains.