- WBPaper00043945:MeHg_regulated_microRNA
Differential expression analysis was conducted for miRNAs with at least 1 RPM expression with DESeq 1.6.1.
MicroRNA with expression level regulated after treatment with Methylmercury (MeHg) by miRNAseq analysis.
- WBPaper00045486:starvation_downregulated
A gene was considered differentially expressed if its fold-changed was greater than 1.5 and it had total reads of more than 10 RPM per comparison.
Genes that showed significantly decreased mRNA expression in starved animals. mRNA samples from N2, rde-4(ne229) and hrde-4(tm1200) animals that were starved or fed for 6 days as L1s were sequenced.
- WBPaper00053420:Microgravity-Simulated_upregulated
Transcriptome Analysis Console version 3.0 software (Affymetrix, CA, USA) for differential gene expression analysis. Selection of differentially expressed genes was basd on filter criteria which were defined as one-way ANOVA p-value of < 0.05 with fold changes of <2 and >2.
Genes that showed significantly increased expression by microgravity simulation using a desktop RPM, from hatching to L4 larva stage.
- WBPaper00053420:Microgravity-Simulated_downregulated
Transcriptome Analysis Console version 3.0 software (Affymetrix, CA, USA) for differential gene expression analysis. Selection of differentially expressed genes was basd on filter criteria which were defined as one-way ANOVA p-value of < 0.05 with fold changes of <2 and >2.
Gene that showed significantly decreased expression by microgravity simulation using a desktop RPM, from hatching to L4 larva stage.
- WBPaper00045486:starvation_upregulated
A gene was considered differentially expressed if its fold-changed was greater than 1.5 and it had total reads of more than 10 RPM per comparison.
Genes that showed significantly increased mRNA expression in starved animals. mRNA samples from N2, rde-4(ne229) and hrde-4(tm1200) animals that were starved or fed for 6 days as L1s were sequenced.
- WBPaper00045217:prg-1_progressively_regulated
Genes with more than 2-fold change in expression level are considered differentially expressed.
Genes altered by more than 2-Fold in late versus early generation prg-1 mutants and prg-1; daf-2 mutants. Samples include prg-1(pk2290), prg-1(n4357), prg-1(tm872), prg-1(pk2290); daf-2(e1368), prg-1(pk2290); daf-2(e1370), prg-1(tm872); daf-2(e1370), prg-1(tm872); daf-2(m41).
- WBPaper00059609:malt-1_ilc-17.1_nfki-1_downregulated
Cufflinks (v2.2.1). q < 0.05.
Transcripts that showed significantly decreased expression in malt-1(db1194);npr-1(ad609) animals, in npr-1(ad609) ilc-17.1(tm5218) animals, and in npr-1(ad609) nfki-1(db1198) animals, comparing to the wild type control npr-1(ad609).
- WBPaper00059609:malt-1_ilc-17.1_nfki-1_upregulated
Cufflinks (v2.2.1). q < 0.05.
Transcripts that showed significantly increased expression in malt-1(db1194);npr-1(ad609) animals, in npr-1(ad609) ilc-17.1(tm5218) animals, and in npr-1(ad609) nfki-1(db1198) animals, comparing to the wild type control npr-1(ad609).
- WBPaper00047132:skn-1(RNAi)_downregulated_glp-1(ts)-dependent
Fold change > 4.0 for glp-1(ts) regulated genes, and fold change < 0.67 for skn-1(RNAi) regulated genes.
Genes with < 0.67 fold decreased expression in skn-1(RNAi) animals comparing to in N2 control at 25 centigrade. Fold change = glp-1(ts) + skn-1 RNAi vs. glp-1(ts) + vector