- WBPaper00055103:thoc-5(wy822)_downregulated
DESeq2, log2 fold change > 2, adjusted p-value < 0.005.
Transcripts that showed significantly decreased expression in FACS sorted neuron cells (labelled by pan-neuronal GFP) from edIs6[unc-119::GFP + rol-6(su1006)]; thoc-5(wy822) comparing to in edIs6.
- WBPaper00055103:thoc-5(wy822)_upregulated
DESeq2, log2 fold change > 2, adjusted p-value < 0.005.
Transcripts that showed significantly increased expression in FACS sorted neuron cells (labelled by pan-neuronal GFP) from edIs6[unc-119::GFP + rol-6(su1006)]; thoc-5(wy822) comparing to in edIs6.
- WBPaper00046499:AMPK_regulated
Genes were tested for differential expression between each mutant strain and wild-type using edgeRs glm method. Briefly, negative binomial models were fitted and dispersion estimates obtained. These were then used to calculate average levels of change between conditions and determine differential expression, using the generalized linear model likelihood ratio test. For each comparison, genes with less than 5 CPM were filtered and those with at least 50% change and False Discovery Rate (FDR) of 1% or less were considered differentially expressed.
mRNAs that showed differential expression in activated AAK-2(uthIs202[aak-2 (intron 1)::aak-2(aa1-aa321)::Tomato::unc-54 3'UTR + rol-6(su1006)]), activiated CRTC-1 (uthEx222[crtc-1p::crtc-1 cDNA (S76S, S179A)::tdTomato::unc-54 3'UTR + rol-6(su1006)]), or AAK-2;CRTC-1 comparing to in N2.
- WBPaper00065315:mating_downregulated_Day6-7
DESeq2 v.1.10.1, fold change > 2, FDR < 0.05.
Transcripts that showed significantly decreased expression in sterile glp-1(e2144) animals after exposed to males during day 6 to day 6 adult stage.
- WBPaper00065315:mating_upregulated_Day6-7
DESeq2 v.1.10.1, fold change > 2, FDR < 0.05.
Transcripts that showed significantly increased expression in sterile glp-1(e2144) animals after exposed to males during day 6 to day 6 adult stage.