- WBPaper00037901:GLD-1_mRNA_targets
Authors calculated the mean IP enrichment from the two analyses and given a cutoff of three-fold, they identified 948 reproducibly enriched mRNAs (14.2%) from of a total of 6635 detected by the array.
948 reproductively enriched mRNAs that co-immunoprecipitate with GLD-1. To identify GLD-1 mRNA targets, authors performed immunoprecipitation (IP) of GLD-1, followed by microarray analysis of the co-IPed mRNAs (RIP-chip). Extracts from young adult transgenic worms expressing a rescuing FLAG and GFP-tagged GLD-1, hereafter referred to as tagged GLD-1, were subjected to IP in triplicate with anti-FLAG (aFLAG IP) or anti-MYC (aMYC IP) antibodies as controls. Comparison of aFLAG IP versus aMYC IP to input revealed a large population of GLD-1-associated transcripts. Authors additionally performed complementary aFLAG IPs upon worms expressing either tagged GLD-1(GGF IP) or non-tagged GLD-1(N2 IP). Comparing transcript 'IP-enrichment values' from both approaches revealed a correlation of 0.96, which indicated high reproducibility of GLD-1 association with specific mRNAs even on a quantitative level.
- WBPaper00050094:glp-1(gf)_upregulated
Fold change > 2.
Transcripts that showed significantly increased expression in the dissected gonads of gld-2(q492) gld-1(q485) (I); glp-1(ar202)(III) (glp-1(gf) NOTCH ON), comparing to in the dissected gonads of gld-2(q492) gld-1(q485) (I); glp-1(e2144) (glp-1(lf) NOTCH OFF)
- WBPaper00050094:glp-1(gf)_downregulated
Fold change > 2.
Transcripts that showed significantly decreased expression in the dissected gonads of gld-2(q492) gld-1(q485) (I); glp-1(ar202)(III) (glp-1(gf) NOTCH ON), comparing to in the dissected gonads of gld-2(q492) gld-1(q485) (I); glp-1(e2144) (glp-1(lf) NOTCH OFF)
- WBPaper00059440:LAG-1_repressed_48hr-auxin
edgeR fold change >= 2, FDR <= 0.05.
Transcripts repressed by lag-1(oz536oz537), in the gld-2(q497)gld-1(q485); glp-1(ar202) background at young adult stage after 48 hours of 1mM auxin treatment.
- WBPaper00059440:LAG-1_activated_48hr-auxin
edgeR fold change >= 2, FDR <= 0.05.
Transcripts activated by lag-1(oz536oz537), in the gld-2(q497)gld-1(q485); glp-1(ar202) background at young adult stage after 48 hours of 1mM auxin treatment.
- WBPaper00059440:LAG-1_activated_2hr-auxin
edgeR fold change >= 2, FDR <= 0.05.
Transcripts activated by lag-1(oz536oz537), in the gld-2(q497)gld-1(q485); glp-1(ar202) background at young adult stage after 2 hours of 1mM auxin treatment.
- WBPaper00059440:LAG-1_activated_4hr-auxin
edgeR fold change >= 2, FDR <= 0.05.
Transcripts activated by lag-1(oz536oz537), in the gld-2(q497)gld-1(q485); glp-1(ar202) background at young adult stage after 4 hours of 1mM auxin treatment.
- WBPaper00059440:LAG-1_repressed_4hr-auxin
edgeR fold change >= 2, FDR <= 0.05.
Transcripts repressed by lag-1(oz536oz537), in the gld-2(q497)gld-1(q485); glp-1(ar202) background at young adult stage after 4 hours of 1mM auxin treatment.
- WBPaper00059440:LAG-1_repressed_2hr-auxin
edgeR fold change >= 2, FDR <= 0.05.
Transcripts repressed by lag-1(oz536oz537), in the gld-2(q497)gld-1(q485); glp-1(ar202) background at young adult stage after 2 hours of 1mM auxin treatment. No gene was found.
- WBPaper00044501:gld-1_let-7_regulated
N.A.
Proteins that showed differential expression in (B) let-7(mg279);[let-7 sponge] when comparing to (A) [let-7 sponge], and in (C) gld-1(op236); let-7(mg279);[let-7 sponge] when comparing to (A) [let-7 sponge]