- WBPaper00053782:daf-2(RNAi)_oocyte_downregulated
One-class SAM analyses (FDR = 1%) were performed in RStudio to identify day 8 daf-2(RNAi) oocyte targets.
Transcripts that showed significantly decreased expression in oocytes isolated from day 8 adult hermaphrodites of daf-2(RNAi);fem-1(hc17) animals, comparing to fem-1(hc17) on control vector animals.
- WBPaper00053782:daf-2(RNAi)_oocyte_upregulated
One-class SAM analyses (FDR = 1%) were performed in RStudio to identify day 8 daf-2(RNAi) oocyte targets.
Transcripts that showed significantly increased expression in oocytes isolated from day 8 adult hermaphrodites of daf-2(RNAi);fem-1(hc17) animals, comparing to fem-1(hc17) on control vector animals.
- WBPaper00065032:PAO1_vs_Delt.Pf4-infection_downregulated
Fold change > 2, p-value < 0.05.
Proteins that showed significantly decreased expression in rrf-3(-);fem-1(-) animal infected P. aeruginosa PAO1 exposure for two days, comparing to in animals exposed to PAO1 infected by the filamentous bacteriophage Pf4.
- WBPaper00057288:Cold_downregulated_protein
Two-tailed Student's t-test (n=3, P-value <0.05 was considered significant).
Proteins that showed significantly decreased expression in CF512 (fer-15(b26)II;fem-1(hc17)IV) treated with empty RNAi vector at 15C comparing to at 20C.
- WBPaper00065032:PAO1_vs_Delt.Pf4-infection_upregulated
Fold change > 2, p-value < 0.05.
Proteins that showed significantly increased expression in rrf-3(-);fem-1(-) animal infected P. aeruginosa PAO1 exposure for two days, comparing to in animals exposed to PAO1 infected by the filamentous bacteriophage Pf4.
- WBPaper00054479:L.fermentum_upregulated
fold change > 5 and p-value < 0.05
Transcripts that showed more than 5 fold increase of expression in fer-15;fem-1 worms after fed by Lactobacillus fermentum strain JDFM216 for 24 hours compared to Escherichia coli OP50 fed control.
- WBPaper00057288:iff-1(RNAi)_upregulated_protein
Two-tailed Student's t-test (n=3, P-value <0.05 was considered significant).
Proteins that showed significantly increased expression in iff-1(RNAi) animals comparing to CF512 (fer-15(b26)II;fem-1(hc17)IV) animals applied with control vector at 15C.
- WBPaper00045401:N.parisii_40h_upregulated
Differentially expressed transcripts were identified using the edgeR Bioconductor package (Empirical analysis of digital gene expression data in R, v 3.0.8). FDR cutoff was set to < 0.05, which yielded lists of genes with > 4-fold difference in expression.
Genes that showed significantly increased expression 40 hours after infection by Nematocida parisii in fer-15(b26); fem-1(hc17), according to RNAseq.