In wild-type larvae, LET-23 RTK is first expressed in the vulval precursor cells in the early L2 stage. LET-23 staining is observed throughout the basolateral membrane of all six vulval precursor cells. In five of the vulval precursor cells (P3.p, P4.p, P5.p, P7.p, and P8.p), little or no LET-23 is present in the apical membrane domain. In the remaining vulval precursor cell (P6.p), LET-23 is present in both the basolateral and apical membrane domains. As P6.p divides in early L3, LET-23 staining continues to be present in both the basolateral and apical membrane domains.
All the vulval precursor cells and their descendants, vulA-vulF, expressed GFP intensely throughout development. No intense expression was detected in hyp7.
In late L3 larvae, LIN-10 is present in descendants of the vulval precursor cells. Not detected LIN-10 staining in the vulval precursor cells in L2 or early L3 larvae, possibly because strong LIN-10 staining in adjacent ventral cord neurons obscures low-level LIN-10 expression in the vulval precursor cells at this stage. LIN-10 is expressed at high levels in neurons, including ring neurons, ventral cord neurons, dorsal cord neurons, lateral neurons, and neurons in the tail.
UNC-62(7b) was expressed in neurons, the ventral nerve cord, vulval precursor cells, and hypodermis beginning in embryos and continuing through adulthood.
hlh-29 is expressed in most cells of the spermatheca of L4 and adult animals, and in the vulva muscles, but not in the spermatheca or vulval precursor cells of younger animals.
Animals carrying this transgene express YFP in many cells throughout development and in the adult, including the vulval precursor cells, hypodermis, intestine, neurons and the body wall muscles.
Expression is observed from late embryogenesis to adult in seam cells, pharynx and intestine, vulva and vulval precursors, gonad distal tip cells, and muscles of the body wall, head and rectum.
Expression is observed in three types of cells: in P-cells from mid-embryo to L2; in vulval precursors at L2 only; in ventral nerve cord from late embryo to adult.