UNC-45 function. In this transgenic line, GFP expression is detected in all muscle cells examined, including body wall muscle cells, pharyngeal muscle cells, anal-intestinal muscle cells, gonad sheath muscle cells, and sex-specific muscle cells in both males and hermaphrodites. This supports a general role of UNC-45 in development and function of all muscles.
vps-45::EGFP is ubiquitously expressed in all major tissues, such as neuron, muscle, hypodermis and intestine. vps-45::EGFP is also expressed in coelomocytes.
YFP-LIN-45 expressed from fosmid[yfp-lin-45] was evident in the cytoplasm of all VPCs. In contrast, at the Pn.px and later stages, YFP-LIN-45 was markedly reduced in the descendants of P6.p compared with the descendants of P5.p and P7.p.
Double-antibody staining against SYD-2 and UNC-10 revealed that SYD-2 colocalizes with UNC-10 as discrete puncta of ~200-400 nm in diameter along the dorsal, ventral, and lateral nerve cords. Double-antibody staining against SYD-2 and UNC-13 revealed that SYD-2 also colocalizes with UNC-13. In both cases, the degree of colocalization was very high but not complete [92.9 +/- 2.3% for UNC-10 (n = 5) and 83.1 +/- 6.2% for UNC-13 (n = 6)]. Some SYD-2 puncta appear to stain with varying amounts of UNC-10 or UNC-13 and vice versa, and in some cases, SYD-2 puncta do not costain at all with UNC-10 or UNC-13.
We compared the expression pattern of unc-79 with unc-80 by expressing GFP driven by 2.7-kb promoter regions for each gene. Fluorescence patterns overlapped throughout the nervous syste, suggesting that UNC-79 and UNC-80 proteins may function together in the same neurons.
We compared the expression pattern of unc-79 with unc-80 by expressing GFP driven by 2.7-kb promoter regions for each gene. Fluorescence patterns overlapped throughout the nervous syste, suggesting that UNC-79 and UNC-80 proteins may function together in the same neurons.