Expression of transcriptional
ttbk-3-7 fusion genes was weak and variable, but was observed primarily in neurons, including subsets of sensory neurons in the amphid sense organs of the head. In particular,
ttbk-3,
ttbk-4 and
ttbk-5 transcriptional fusion genes were expressed in either or both of the pheromone-sensing ASK and ASI sensory neurons. Additional expressing neurons could not be reliably identified due to variable and weak expression of these reporter genes.Functional GFP-tagged TTBK-3 and TTBK-4 fusion proteins were localized throughout the cell in ASK, but appeared to be excluded from sensory cilia.