The expression of
nhr-208 GFP reporters started in embryos at the 1.5-fold stage within the pharynx, intestinal sphincter, and epidermal cells in the tail. By the 3-fold stage of embryogenesis, additional expression was observed in rectal gland and surrounding cells. During all larval stages, strong expression of the transgenes was visible in pharyngeal and unidentified head neurons, the pharyngeal-intestinal valve cell, the posterior part of the intestine, the intestinal sphincter, two rectal gland cells, the intestinal-rectal valve cell, and the epidermal
hyp10 cell. In males, the expression was seen in several rays (6-8) and other male specific neurons.