SMU-2::GFP is a nuclear protein. SMU-2::GFP was observed at all stages of development including early embryogenesis and in oocytes. To show that SMU-2 is ubiquitously expressed, embryos and larvae carrying the integrated
smu-2::gfp array were costained with the DNA stain DAPI and SMU-2::GFP was observed in essentially every cell that was stained by DAPI. Unlike most transgenes in C. elegans, the
smu-2::gfp transgene was expressed in the germ line. To confirm this result with another SMU-2-expressing transgene, a rescuing transgene that placed a HA tag at the very end of the protein were constructed. This construct also led to nuclear expression in all cells, including the germ line.