In contrast to hermaphrodites,
lgc-35 is not expressed in any of the male enteric muscles, including the sphincter muscle, suggesting that it does not play a role in defecation in adult males. Like hermaphrodites, adult males expressed
lgc-35 in acetylcholine motor neurons, the head mesodermal cell, and in the head interneurons AIY and AVD. In the male tail,
lgc-35 is expressed in sensory rays 2, 3, 4, 5, and 9, the oblique muscle, the SPV neurons, and possibly the SPD neuron.