Confocal laser microscopy showed RAD-8::GFP fluorescence in various tissues that include the neurons, pharynx, muscles, and intestine. Thus, RAD-8 is likely ubiquitously expressed in C. elegans.
Immunostained whole mounts of WT animals demonstrated that LIN-54 was present in the nuclei of all or almost all cells from the embryo through the adult. LIN-54 was not detected in lin-54(n3423) mutant animals.
RAD-23 expression was detected throughout the animal at the young adult (L4 + 1 d) stage, including neuronal cell bodies and processes, the vulva, the nerve cord, and throughout the head.
miR-54-56 GFP reporter was restricted in expression to anterior and ventral cells identified as neurons, tail hypodermal cells, and cells of the excretory system.
miR-54-56 GFP reporter was restricted in expression to anterior and ventral cells identified as neurons, tail hypodermal cells, and cells of the excretory system.