Expr13520

Extremely faint expression was only observed in the posterior pharynx. To amplify the PQN-75:: GFP::3xFLAG signal, worms were fixed and stained green with M2 anti-flag and a blue DAPI/DNA costain, and still there was no evidence of germline expression. PQN-75 staining was exclusively in the pharynx, starting in the threefold stage of embryogenesis, becoming progressively more pronounced through larval development. Within the pharynx, PQN-75 was most abundant in the pharyngeal gland cells and could be observed in gland-cell processes that extend along the pharyngeal lumen. Interestingly, PQN-75 staining was not detected within the pharyngeal lumen, buccal cavity, or on the anterior cuticle. Instead, 77% (n=200) of larval-staged worms had varying amounts of PQN-75 aggregates throughout the pharynx, suggesting that gland cells secrete PQN-75 into the surrounding pharyngeal muscle.

Expr12490

pqn-57 was expressed in pharyngeal muscle and near the rectum. Expression of the pqn-57 reporter persisted in adult pharynxes.

Expr12189

pqn-73 is expressed in the arcade cells.

Expr13390

GFP::3xFLAG:: PQN-59 expression was ubiquitous and cytoplasmic.

Expr16042

Both PQN-59 and GTBP-1 are expressed throughout development, and are widely expressed in adult C. elegans animals, including in the germline and the embryo.

Expr16039

Both PQN-59 and GTBP-1 are expressed throughout development, and are widely expressed in adult C. elegans animals, including in the germline and the embryo.

Expr9722

Expression becomes detectable around the comma stage of embryogenesis and persists through adulthood. Expression in vulval precursor cells is strong and can first be seen in L3. PQN-47::GFP is expressed in seam cells, peaking at L2 and ceasing after the seam cells differentiate in late L4, concurrent with the appearance of alae. The intestine shows variably undetectable to low pqn-47 expression (always less than in the neurons) and gets dimmer as development progresses, especially after L3. The two bulbs of the pharynx, specifically pharyngeal muscle cells pm3-8 (not pm6), are variably bright. Overall expression levels are lower in adults than younger animals, with only some expression in head and tail neurons remaining.

Expr9990

A 13-kb DNA fragment derived from the pqn-41 locus and fused to gfp sequences is broadly expressed in transgenic animals in most cells starting in the embryo. However, expression is only switched on in the linker cell as the cell begins to die. A 2.5-kb DNA subfragment derived from this reporter promotes gfp expression nearly exclusively in the linker cell, and only upon cell death initiation.

Expr9754

Nuclear-localized GFP was seen from embryo to adult, in many components. There was considerable variation between individuals of each transgenic line generated which may reflect the very faint GFP expression, albeit stronger than for any of the strains generated for fUL#JW170, with gfp inserted directly upstream of the pqn-21 stop codon. In UL3453, bodywall muscle as well as intestinal and hypodermal GFP expression was observed. In UL3419, which expressed at a lower level than UL3453, GFP expression was seen in bodywall muscle and spermathecae. GFP in bodywall muscle was not always very nuclear-localized.

Expr8169

At hatching, hlh-3 is expressed in most of the neurons of the nerve ring ganglia. This expression is undetectable by the early L1 larval stage. The most conspicuous expression during L1 is within the P cell lineages. Specifically, hlh-3 is expressed in the ectodermal-like P cells, and expression persists in the primary and secondary neural precursors of the P cell lineages. In addition, it appears to be expressed in all 53 of the resulting postmitotic motor neurons. Expression in most of these neurons is undetectable by the L2 larval stage. It is worth noting here that these neurons represent 77% of all motor neurons mediating locomotion. hlh-3 is also expressed in the HSNs and ventral type C (VC) motor neurons throughout larval development. At hatching, expression in the HSNs is weak, but peaks at the L3 larval stage and persists until the late L4 larval stage. Expression in the VCs also peaks during L3. Overall, hlh-3 appears to be expressed in all neural precursors. Based on expression in the P cell lineages, expression generally persists through terminal cell division and disappears from postmitotic neurons shortly thereafter. However, hlh-3 is also expressed in non-neural cells such as the P cells, as well as postmitotic, differentiating neurons such as the HSNs and VCs.