We found that an endogenous
php-3::GFP knock-in we created via CRISPR/Cas9-mediated gene editing was expressed in DVA, DVC, PVR, PHCL/R, and PLNL/ R neurons, whereas a translational reporter stIs10286[
nob-1::GFP] was expressed only in DVA, PHCL/R, and PLNL/R neurons, suggesting that
php-3 and
nob-1 may have slightly different expression patterns. Neither
php-3 nor
nob-1 was expressed in the ventral nerve cord.