Pcdc-25.4::gfp was expressed in many neurons, including pharyngeal neurons, the ventral nerve cord, and tail neurons in both hermaphrodites and males. However, the signal was not detected in the dorsal nerve cord. Pcdc-25.4::gfp expression was also observed in pharyngeal muscles, the head ganglion, preanal ganglion, and lumbar ganglion. In particular, Pcdc-25.4::gfp was expressed in ray cell bodies, spicule neurons, hook neurons, and postcloacal sensilla neurons in male tails.
cdc-25.4 was also expressed in germ cells, including oocytes and sperm.