HSP-25 and HSP-12.1 were localized to the M-lines and dense bodies of the body wall muscles, as previously reported (Ding and Can- dido, 2000a; Meissner et al., 2011). Y55F3BR.6 displayed expression patterns similar to those of HSP-25 and HSP-12.1. Except for the signals leaking from the neighboring body-wall muscle cells, no expression of the three sHSPs was observed in the epidermis.
HSP-25 and HSP-12.1 were localized to the M-lines and dense bodies of the body wall muscles, as previously reported (Ding and Can- dido, 2000a; Meissner et al., 2011). Y55F3BR.6 displayed expression patterns similar to those of HSP-25 and HSP-12.1. Except for the signals leaking from the neighboring body-wall muscle cells, no expression of the three sHSPs was observed in the epidermis.
HSP-25 and HSP-12.1 were localized to the M-lines and dense bodies of the body wall muscles, as previously reported (Ding and Can- dido, 2000a; Meissner et al., 2011). Y55F3BR.6 displayed expression patterns similar to those of HSP-25 and HSP-12.1. Except for the signals leaking from the neighboring body-wall muscle cells, no expression of the three sHSPs was observed in the epidermis.
During L4 lethargus, hsp-4 was notably expressed in the epithelial seam of undisrupted animals. We observed that hsp-4 expression in seam cells coincided with the generation of the alae - the adult cuticular ridges.
Expression of hsp-6 was broad and most clearly visible in the intestine. Strong expression of hsp-6 or an accumulation of the reporter led to bright staining of a posterior segment of the intestine.
HSP-4::GFP was exclusively localized in the PVD soma, colocalizing with a rough ER marker TRAM, HSP-4's endoplasmic reticulum localization pattern is consistent with the observation that its mammalian homolog BiP is localized in rough endoplasmic reticulum.
HSP-43 was highly and exclusively expressed in the epidermis and displayed distinct parallel short stripe patterns characteristic of C elegans hemidesmosomes.