HRP-2 is expressed in all cells of the early embryo commencing between the 28- and 64-cell stages, with staining of the large gut cell nuclei being particularly prominent by the 3-fold stage of development. This ubiquitous pattern of expression continues throughout larval development and into the adult worm. As transgenes are typically poorly expressed in the germline, the gonad was dissected from adult worms and found to be clearly reactive with R64 Ab. Antibody staining also confirmed expression of HRP-2 in the large nuclei of the gut cells. No staining was observed with the preimmune serum.
Asymmetric expression of imb-2 in AWCON was also observed in our initial assessment of the imb-2 expression pattern using imb-2 fosmid::GFP in which we fused GFP with the C terminus of IMB-2 by homologous recombination, that rescued imb-2(vy10) mutants.