The expression of CEH-23::GFP was restricted to a handful of neurons and the intestine of the worm. Although the neurons in which CEH-23 is expressed have not been identified, the observed neuronal expression of the CEH-23::GFP construct resembles that of a previously published GFP construct fused to a partial CEH-23 (gmIs18[
ceh-23::gfp]), which was reported to express in the pair of CAN neurons in the central body and 12 sets of sensory neurons in the head (10 pairs) and the tail (2 pairs). While the neuronal expression of CEH-23 was found in all the transgenic worms, the intestinal expression was only detected in ,50% of the transgenic worms with a stronger expression in the posterior intestine.