The CEH-30::GFP fusion is expressed widely during embryonic development, and the expression is especially robust in the anterior half of the embryos. CEH-30::GFP expression continues into the larval and adult stages, albeit at greatly reduced levels. In hermaphrodite smIs54 embryos, CEH-30::GFP was never seen in ventral CEMs (0/15 embryos). In contrast, in
tra-1(
n1099); smIs54 XX embryos, which are completely masculinized and virtually identical to male embryos, CEH-30::GFP was observed in all ventral CEMs (15/15 embryos), suggesting that CEH-30 is expressed in developing male CEM neurons.