An extrachromosomal array containing 1.6 kb of the presumptive C46E1.3 promoter linked to gfp is strongly expressed both in the excretory canal cell and in the glial amphid sheath cells. Expression within the canal cell begins soon after the cell is born, and persists throughout the lifetime of the animal. A translational construct expressing C46E1.3 cDNA linked to GFP solely within the canal and head mesodermal cell is strongly expressed within the canal cytoplasm.
Under normal osmolarity conditions, AQP-8::GFP localized within the excretory cell soma and throughout the entire anterior excretory canal. Within the posterior excretory canal, the signal intensity decreased from the soma toward the tail region, although the canal is fully extended throughout the whole body in adults (Khan et al., 2013; Kolotuev et al., 2013). In most animals, the signal was only visible along ~80% of the length of the posterior canal.
exc-5 5'::gfp was expressed in the excretory canal. exc-5::gfp was observed in excretory canal cells as well as pharyngeal muscles, rectal epithelial cells, and several head and tail neurons.