The Arf-6::gfp promoter construct resulted in very early expression, similar to that seen with Arf-1::GFP. Expressed in the ventral nerve cord, vulva, intestine, and body wall and pharyngeal muscle tissues. Arf-6::GFP was in the excretory cell.
Arf-3::GFP was only seen at later stage embryos (>200 cells). Expressed in the ventral nerve cord, vulva, intestine, and body wall and pharyngeal muscle tissues. Arf-3::GFP was in seam cells.
Arf-1::GFP or Arf1::gfp expression was first detectable in very young embryos with as few as 48-64 cells. Expressed in the ventral nerve cord, vulva, intestine, and body wall and pharyngeal muscle tissues. Arf-1::GFP was clearly seen in the uterus, spermatheca, and gonadal sheath,
Asymmetric expression of imb-2 in AWCON was also observed in our initial assessment of the imb-2 expression pattern using imb-2 fosmid::GFP in which we fused GFP with the C terminus of IMB-2 by homologous recombination, that rescued imb-2(vy10) mutants.