The Arf-6::gfp promoter construct resulted in very early expression, similar to that seen with Arf-1::GFP. Expressed in the ventral nerve cord, vulva, intestine, and body wall and pharyngeal muscle tissues. Arf-6::GFP was in the excretory cell.
In vivo examination of the PFK-1.1 expression pattern revealed, as expected, broad expression of PFK-1.1 in multiple tissue types, including muscles and neurons.
Arf-3::GFP was only seen at later stage embryos (>200 cells). Expressed in the ventral nerve cord, vulva, intestine, and body wall and pharyngeal muscle tissues. Arf-3::GFP was in seam cells.
Arf-1::GFP or Arf1::gfp expression was first detectable in very young embryos with as few as 48-64 cells. Expressed in the ventral nerve cord, vulva, intestine, and body wall and pharyngeal muscle tissues. Arf-1::GFP was clearly seen in the uterus, spermatheca, and gonadal sheath,
Expression of cDJR-1.1::GFP was detected exclusively in the intestine. The major expression sites of cDJR-1.1 and cDJR-1.2 do not overlap and are fairly consistent throughout the whole larval and adult stages of worms.