AAK-2c was expressed in various tissues, including the pharynx, intestine, muscles, hypodermis, and neurons, consistent with previous AAK-2 expression.
During L1 diapause, Paak-2::aak-2::gfp was highly expressed in most tissues including the intestine, excretory canal, pharynx, somatic gonad, neurons, hypodermis, and body wall muscle.
aak-2 is widely and strongly expressed during dauer. An aak-2p::AAK-2N::GFP truncated translational fusion protein is expressed in pharyngeal muscles, nervous system, coelomocytes, hypodermis, excretory cell, muscles, intestine, whole animal.
In the transgenic C. elegans line expressing the AAK-2::GFP fusion protein, expression was observed in the pharynx, the ventral cord, other neurons including the hermaphrodite-specific neuron (HSN), body wall muscles, the vulva, the excretory canal, and weakly in the intestine. GFP expression was also seen in the distal tip cells, spermatheca, and sheath cells.
Asymmetric expression of imb-2 in AWCON was also observed in our initial assessment of the imb-2 expression pattern using imb-2 fosmid::GFP in which we fused GFP with the C terminus of IMB-2 by homologous recombination, that rescued imb-2(vy10) mutants.