Consistent with previously reported
bed-3::gfp reporter expression,
bed-3 mRNA (smFISH) was predominantly expressed in hypodermal cells and vulval cells. We next examined whether
bed-3 is expressed earlier in the M lineage using ayIs6[
hlh-8::gfp]. We found that
bed-3 is expressed in M daughters and descendants as well as SM cells. In L1 and L2 stages, the
bed-3 smFISH signal appeared stronger in 2 M and 4 M stages compared to 8 M, 16 M and 18 M stages. In the L3 stage,
bed-3 is expressed in SM cells near the vulval region of the animal.