MPK-1A was present in both GL+ and GL- animals, but MPK-1B protein was found only in GL+ animals. Thus mpk-1b RNA and its MPK-1B protein are predominantly expressed in the germline.
SKN-1B::GFP is expressed in ASI neurons. DiI staining confirms SKN- 1B::GFP in the ASI neurons and identified two of these additional neurons as being the ADLs.
TTM-1B::GFP was expressed in multiple tissues including intestine, head neurons, seam cells, hypodermis, and the vulva consistent with the tissue distribution pattern displayed by Pttm-1b::GFP transgenic animals.
The ptb-1b promoter drove strong expression in neurons. In the nervous system, the ptb-1a and ptb-1b promoters drove the expression in distinct but partially overlapping neuron classes, including ASE.
The mpk-1ab mRNA was abundant in both GL+ and GL- animals, but mpk-1b mRNA was very low or undetectable in GL- animals. Therefore, mpk-1b appears to be enriched in the germline.