TOP-1a protein was localized to the neuronal cells as had been observed in the late embryos and also excretory cells. Although Ptop-1::GFP expression in neuron appears to correlate with the neuronal localization of TOP-1a, GFP and TOP-1a did not overlap exactly.
The ALP-1A::GFP is expressed early in muscle development where it first appears during the comma-stage in the characteristic pair of bands that line the lateral surfaces of the embryo, demarcating the nascent body wall muscle cells. Throughout adulthood ALP-1A is most prevalent in the body wall muscle. In addition to the muscle expression, ALP-1A::GFP is expressed in the embryonic hypodermis.
Imaging this strain revealed significant differences in SNT-1 isoform expression and localization. The SNT-1A isoform is abundantly expressed in most neurons, and the distribution of this isoform is synaptic, resembling that of the endogenous SNT-1 (see Expr334). In contrast, the SNT-1B isoform is expressed at lower levels and in fewer neurons than SNT-1A. Many neurons express both SNT-1A and -1B, although the relative amount of each isoform varies greatly between individual cells, and some neurons appear to express only the SNT-1A isoform.