The DAF-28::mCherry expression followed the reported expression pattern of
pdaf-28::GFP transcriptional reporter, with fluorescent protein detected in head and tail neurons, pharynx, hypodermis, and other tissues. DAF-28::mCherry was efficiently secreted upon expression in L1 stage, as detected by its uptake by endocytic scavenger cells, coelomocytes. Interestingly, compared to the DAF-28::mCherry fusion protein, the previously described DAF-28::GFP protein was not efficiently secreted, as judged by coelomocyte fluorescence, and instead appeared to accumulate in neuronal cell bodies. This may be due to misfolding of the GFP moiety.