The transcriptional
nlp-26 reporter was dynamically expressed in the VPCs and their sister Pn.a neurons in the VNC. In early to mid-L2 larvae, Pnlp-26-nls::lacZ::gfp was expressed in all VPCs and their sister Pn.a neurons. In addition,
nlp-26 was strongly expressed in the
hyp7 cell at all stages. By the late L2/early L3 stage,
nlp-26 transcription was upregulated in P6.p and the P6.a neurons, while expression faded in the other VPCs.
nlp-26 continued to be expressed in the P6.px daughter cells of mid-L3 larvae. Note that descendants of the 3° VPCs P3.p, P4.p and P8.p began to express
nlp-26 after they had fused with
hyp7.