We examined the expression and localization of endogenous NEKL-4 by using CRISPR/Cas9 to engineer nekl-4
::mneongreen and nekl-4
::mscarlet strains. These CRISPR-generated reporters use the nekl-4
locus as well as a short flexible linker, and have significantly dimmer fluorescence than the extrachromosomal nekl-4p
::gfp array [Expr15275]. With both extrachromosomal and CRISPR reporters, NEKL-4 was observed in the same neurons and similar subcellular locations.