COL-19::GFP expression was also examined in dauer stage larvae. After allowing TP12:kaIs(
col-19::gfp) strain to starve, a large population of dauer larvae were obtained, examined, and found not to express the COL-19::GFP protein in their cuticles. Likewise, no annuli or ala-specific GFP expression was detected in temperatureinduced dauers of a
daf-2(
e1370) mutant allele strain crossed with TP12:kaIs(
col-19::gfp) males.